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CycA-Venus-Flag (1305)
(Plasmid #39853)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 39853 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 39853-D50 50 μg of DNA in Tris buffer $495
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DNA 39853-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pcDNA5/FRT/TO
  • Backbone manufacturer
    Pines lab
  • Modifications to backbone
    First, Venus-Flag was added to pcDNA5/FRT/TO between BstXI and XhoI. Second, an attR1-CmR-ccdB-attR2 GATEWAY cassette was inserted into at the AflII site.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cyclin A
  • Species
    H. sapiens (human)
  • Entrez Gene
    CCNA2 (a.k.a. CCN1, CCNA)
  • Tags / Fusion Proteins
    • Venus (C terminal on backbone)
    • Flag (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CycA-Venus-Flag (1305) was a gift from Jonathon Pines (Addgene plasmid # 39853 ; http://n2t.net/addgene:39853 ; RRID:Addgene_39853)

  • For your References section:

    How cyclin A destruction escapes the spindle assembly checkpoint. Di Fiore B, Pines J. J Cell Biol. 2010 Aug 23;190(4):501-9. 10.1083/jcb.201001083 PubMed 20733051
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