|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16654||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5200
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Gene/Insert nameCyclin E
SpeciesH. sapiens (human)
Insert Size (bp)2000
Entrez GeneCCNE1 (a.k.a. CCNE, pCCNE1)
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
A fragment of human cyclin E cDNA including the entire ORF was cloned into pBI-EGFP.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Cyclin E-pBI-EGFP was a gift from Bert Vogelstein (Addgene plasmid # 16654 ; http://n2t.net/addgene:16654 ; RRID:Addgene_16654)
For your References section:Inactivation of hCDC4 can cause chromosomal instability. Rajagopalan H, Jallepalli PV, Rago C, Velculescu VE, Kinzler KW, Vogelstein B, Lengauer C. Nature. 2004 Mar 4. 428(6978):77-81. 10.1038/nature02313 PubMed 14999283