Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


CycA-Venus-Flag (1305)
(Plasmid #39853)

Full plasmid sequence is not available for this item.



Item Catalog # Description Quantity Price (USD)
Plasmid 39853 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Pines lab
  • Modifications to backbone
    First, Venus-Flag was added to pcDNA5/FRT/TO between BstXI and XhoI. Second, an attR1-CmR-ccdB-attR2 GATEWAY cassette was inserted into at the AflII site.
  • Vector type
    Mammalian Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    Cyclin A
  • Species
    H. sapiens (human)
  • Entrez Gene
    CCNA2 (a.k.a. CCN1, CCNA)
  • Tags / Fusion Proteins
    • Venus (C terminal on backbone)
    • Flag (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CycA-Venus-Flag (1305) was a gift from Jonathon Pines (Addgene plasmid # 39853 ; ; RRID:Addgene_39853)
  • For your References section:

    How cyclin A destruction escapes the spindle assembly checkpoint. Di Fiore B, Pines J. J Cell Biol. 2010 Aug 23;190(4):501-9. 10.1083/jcb.201001083 PubMed 20733051