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CycA-Venus-Flag (1305)
(Plasmid #39853)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 39853 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA5/FRT/TO
  • Backbone manufacturer
    Pines lab
  • Modifications to backbone
    First, Venus-Flag was added to pcDNA5/FRT/TO between BstXI and XhoI. Second, an attR1-CmR-ccdB-attR2 GATEWAY cassette was inserted into at the AflII site.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cyclin A
  • Species
    H. sapiens (human)
  • Entrez Gene
    CCNA2 (a.k.a. CCN1, CCNA)
  • Tags / Fusion Proteins
    • Venus (C terminal on backbone)
    • Flag (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CycA-Venus-Flag (1305) was a gift from Jonathon Pines (Addgene plasmid # 39853 ; http://n2t.net/addgene:39853 ; RRID:Addgene_39853)
  • For your References section:

    How cyclin A destruction escapes the spindle assembly checkpoint. Di Fiore B, Pines J. J Cell Biol. 2010 Aug 23;190(4):501-9. 10.1083/jcb.201001083 PubMed 20733051