Blimp1pGL3 (Human Blimp1 promoter in pGL3-basic)
(Plasmid #40340)

• Depositing Lab: Alexander Dent
• Depositing Organization: Indiana University School of Medicine, Indianapolis
  Located in United States of America
• Publication: Vasanwala et al J Immunol. 2002 Aug 15;169(4):1922-9.
• Citations: 1 Article

Backbone

• Vector backbone: pGL3-basic
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4818
• Total vector size (bp): 6818
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Blimp1 promoter
• Alt name: PRDM1 promoter
• Species: H. sapiens (human)
• Insert Size (bp): 2000
• GenBank ID: NG_029115.1
• Entrez Gene: PRDM1 (a.k.a. BLIMP-1, BLIMP1, PRDI-BF1)
• Promoter: Blimp1
• Tag / Fusion Protein:
  • Luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacI (unknown if destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: RVprimer3
• 3′ sequencing primer: LucNrev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A 2000-bp region of the human Blimp-1 promoter was isolated from human (Raji cell) genomic DNA by PCR using 5
-ATTTTAGGGTTGCCTGGCTAAGCC-3
as the forward and 5
-GTCACGGCAGCACTTTGTCTGTGT-3
as the reverse primers. The PCR product obtained was ligated into the pcR2.1 TOPO cloning vector (Invitrogen, Carlsbad, CA) and then subcloned into pGL3-basic (Promega, Madison, WI) using SacI and XhoI sites. The promoter construct was sequenced to verify that the proper sequence was amplified.

DNA (Catalog # 40340-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 40340-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.