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pCMV5-rat ERK2-K52R
(Plasmid #40813)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40813 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 40813-D50 50 μg of DNA in Tris buffer $495
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DNA 40813-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCMV5
  • Backbone manufacturer
    David Russell, UTSW
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 6200
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MAPK1
  • Alt name
    ERK2
  • Alt name
    p42
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1500
  • Mutation
    K52R
  • GenBank ID
    NM_053842
  • Entrez Gene
    Mapk1 (a.k.a. ERK-2, ERT1, Erk2, p42-MAPK)
  • Promoter CMV
  • Tag / Fusion Protein
    • His6 (N terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Melanie Cobb, UTSW

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV5-rat ERK2-K52R was a gift from Natalie Ahn (Addgene plasmid # 40813 ; http://n2t.net/addgene:40813 ; RRID:Addgene_40813)

  • For your References section:

    The gatekeeper residue controls autoactivation of ERK2 via a pathway of intramolecular connectivity. Emrick MA, Lee T, Starkey PJ, Mumby MC, Resing KA, Ahn NG. Proc Natl Acad Sci U S A. 2006 Nov 28;103(48):18101-6. Epub 2006 Nov 17. 10.1073/pnas.0608849103 PubMed 17114285
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