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PBCAG-Cerulean
(Plasmid #40997)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 40997 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    PBCAG-eGFP
  • Backbone size w/o insert (bp) 6336
  • Total vector size (bp) 6426
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cerulean
  • Species
    Synthetic

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site Not1 (not destroyed)
  • 5′ sequencing primer pCAG-F
  • 3′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PBCAG-Cerulean was a gift from Joseph Loturco (Addgene plasmid # 40997 ; http://n2t.net/addgene:40997 ; RRID:Addgene_40997)
  • For your References section:

    A method for stable transgenesis of radial glia lineage in rat neocortex by piggyBac mediated transposition. Chen F, LoTurco J. J Neurosci Methods. 2012 Jun 15;207(2):172-80. Epub 2012 Apr 11. 10.1016/j.jneumeth.2012.03.016 PubMed 22521325