pNC1137
(Plasmid
#41563)
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Depositing Lab
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Depositing OrganizationUniversity of North Carolina at Chapel Hill
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 41563 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 41563-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 41563-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepUC118
- Total vector size (bp) 3119
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameUra3-FUS1-UBIMdkGFP*-SpHis5-Tim9
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SpeciesS. cerevisiae (budding yeast), S. pombe (fission yeast); Aequorea victoria
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Insert Size (bp)3833
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MutationGFP mutations: Phe 64 to Leu, S65 to Thr, V163 to A His 77 codon CAT to CAC; destroys NdeI site
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 41563-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 41563-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNC1137 was a gift from Beverly Errede (Addgene plasmid # 41563 ; http://n2t.net/addgene:41563 ; RRID:Addgene_41563) -
For your References section:
An improved short-lived fluorescent protein transcriptional reporter for Saccharomyces cerevisiae. Houser JR, Ford E, Chatterjea SM, Maleri S, Elston TC, Errede B. Yeast. 2012 Dec;29(12):519-30. doi: 10.1002/yea.2932. Epub 2012 Nov 21. 10.1002/yea.2932 PubMed 23172645
Map uploaded by the depositor.
