pNC1136
(Plasmid
#41562)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 41562 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepUC118
- Total vector size (bp) 3119
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameUra3-FUS1-UBIYdkGFP*-SpHis5-Tim9
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SpeciesS. cerevisiae (budding yeast), S. pombe (fission yeast); Aequorea victoria
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Insert Size (bp)3833
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MutationGFP mutations: Phe 64 to Leu, S65 to Thr, V163 to A His 77 codon CAT to CAC; destroys NdeI site
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNC1136 was a gift from Beverly Errede (Addgene plasmid # 41562 ; http://n2t.net/addgene:41562 ; RRID:Addgene_41562) -
For your References section:
An improved short-lived fluorescent protein transcriptional reporter for Saccharomyces cerevisiae. Houser JR, Ford E, Chatterjea SM, Maleri S, Elston TC, Errede B. Yeast. 2012 Dec;29(12):519-30. doi: 10.1002/yea.2932. Epub 2012 Nov 21. 10.1002/yea.2932 PubMed 23172645
