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pAquaN1
(Plasmid #42888)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42888 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 42888-D50 50 μg of DNA in Tris buffer $495
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DNA 42888-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pECFP-N1
  • Backbone manufacturer
    Clontech
  • Total vector size (bp) 4700
  • Modifications to backbone
    None
  • Vector type
    Mammalian Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Aquamarine
  • Alt name
    ECFP-T66S-H149D
  • Species
    A. victoria
  • Mutation
    T66S and H149G

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAquaN1 was a gift from Fabienne Merola (Addgene plasmid # 42888 ; http://n2t.net/addgene:42888 ; RRID:Addgene_42888)

  • For your References section:

    Minimum set of mutations needed to optimize cyan fluorescent proteins for live cell imaging. Erard M, Fredj A, Pasquier H, Beltolngar DB, Bousmah Y, Derrien V, Vincent P, Merola F. Mol Biosyst. 2013 Feb 2;9(2):258-67. doi: 10.1039/c2mb25303h. Epub 2012 Nov 28. 10.1039/c2mb25303h PubMed 23192565
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