|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||43814||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7711
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
- Promoter CMV
/ Fusion Protein
- V5 (C terminal on insert)
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer TK-pA-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Active Wnt5B-V5 was a gift from Xi He (Addgene plasmid # 43814 ; http://n2t.net/addgene:43814 ; RRID:Addgene_43814)
For your References section:Disulfide Bond Requirements for Active Wnt Ligands. MacDonald BT, Hien A, Zhang X, Iranloye O, Virshup DM, Waterman ML, He X. J Biol Chem. 2014 May 19. pii: jbc.M114.575027. 10.1074/jbc.M114.575027 PubMed 24841207