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Active Wnt11-V5
(Plasmid #43824)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 43824 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.2/V5-DEST
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 7711
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human WNT11
  • Alt name
    HWNT11
  • Species
    H. sapiens (human)
  • Entrez Gene
    WNT11 (a.k.a. HWNT11)
  • Promoter CMV
  • Tag / Fusion Protein
    • V5 (C terminal on insert)

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Active Wnt11-V5 was a gift from Xi He (Addgene plasmid # 43824 ; http://n2t.net/addgene:43824 ; RRID:Addgene_43824)
  • For your References section:

    Disulfide Bond Requirements for Active Wnt Ligands. MacDonald BT, Hien A, Zhang X, Iranloye O, Virshup DM, Waterman ML, He X. J Biol Chem. 2014 May 19. pii: jbc.M114.575027. 10.1074/jbc.M114.575027 PubMed 24841207