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pcDNA3 mHIF-2α MYC (P405A/P530V/N851A)
(Plasmid #44027)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44027 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5446
  • Total vector size (bp) 8070
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    mHIF-2α (P405A/P530V/N851A)
  • Alt name
  • Alt name
    Hypoxia-inducible factor-2alpha
  • Alt name
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • Mutation
    P405A, P530V, N851A
  • GenBank ID
    AK158430.1 AAH57870.1
  • Entrez Gene
    Epas1 (a.k.a. HIF-2alpha, HIF2A, HLF, HRF, MOP2, bHLHe73)
  • Promoter CMV
  • Tag / Fusion Protein
    • Myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-F; T7
  • 3′ sequencing primer BGH-rev; Sp6
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid encodes an oxygen-regulation insensitive (normoxia-stable and active) full-length murine HIF-2α.

Full-length murine HIF-2α cDNA was generated by reverse transcription-PCR and inserted into a pcDNA3 expression vector by using primers incorporated with restriction sites.

A 10-amino-acid c-myc tag was further inserted at the C terminus of the HIF-2α cDNAs by using a Pfu-PCR-based protocol. The same Pfu-PCR protocol was then used to change residues Proline 405, Proline 530 and Asparagine 851. The newly constructed plasmid was sequenced to confirm encoding of the correct protein.

This plasmid has an A94V polymorphism that does not affect protein function.

Alternate plasmid names: HIF-2α triple mutant (TM); pcDNA3 HIF-2α TM

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 mHIF-2α MYC (P405A/P530V/N851A) was a gift from Celeste Simon (Addgene plasmid # 44027 ; ; RRID:Addgene_44027)
  • For your References section:

    The N-terminal transactivation domain confers target gene specificity of hypoxia-inducible factors HIF-1alpha and HIF-2alpha. Hu CJ, Sataur A, Wang L, Chen H, Simon MC. Mol Biol Cell. 2007 Nov;18(11):4528-42. Epub 2007 Sep 5. 10.1091/mbc.e06-05-0419 PubMed 17804822