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pcDNA3-MTS-WT-c-Src-FLAG
(Plasmid #44652)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44652 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 44652-D50 50 μg of DNA in Tris buffer $495
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DNA 44652-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 7260
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1608
  • GenBank ID
    NM_005417
  • Entrez Gene
    SRC (a.k.a. ASV, SRC1, THC6, c-SRC, p60-Src)
  • Promoter CMV promoter
  • Tags / Fusion Proteins
    • mitochondria-targeting sequence (residues 1-40 of the amino acid sequence of very long chain acyl-CoA dehydrogenase) (N terminal on insert)
    • FLAG tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bgl II (not destroyed)
  • 3′ cloning site Xho I (not destroyed)
  • 5′ sequencing primer aaagccacttgcctaggga
  • 3′ sequencing primer cgcctgtgcctagaggtt
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-MTS-WT-c-Src-FLAG was a gift from Yoshimi Homma (Addgene plasmid # 44652 ; http://n2t.net/addgene:44652 ; RRID:Addgene_44652)

  • For your References section:

    Mitochondrial c-Src regulates cell survival through phosphorylation of respiratory chain components. Ogura M, Yamaki J, Homma MK, Homma Y. Biochem J. 2012 Oct 15;447(2):281-9. 10.1042/BJ20120509 PubMed 22823520
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