-
Depositing Labs
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
---|---|---|---|---|---|---|
Plasmid | 13663 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepCMV5 *
- Backbone size w/o insert (bp) 4700
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namesrc
-
Alt namec-src
-
SpeciesM. musculus (mouse)
-
Insert Size (bp)2000
-
Entrez GeneSrc (a.k.a. AW259666, pp60c, pp60c-src)
- Promoter CMV
-
Tag
/ Fusion Protein
- Myr (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer CMV forward primer
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
-
Terms and Licenses
-
Industry Terms
- Not Available to Industry
-
Articles Citing this Plasmid
Depositor Comments
* The depositing lab is not completely sure of the backbone for this plasmid; Addgene's sequencing results indicate that it may instead be pcDNA3. The plasmid should still function correctly, but users should consult Addgene's sequencing results and exercise caution if subcloning is necessary.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCMV5 mouse src was a gift from Joan Brugge & Peter Howley (Addgene plasmid # 13663 ; http://n2t.net/addgene:13663 ; RRID:Addgene_13663)