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pCMV5 mouse src K295R Y527F
(Plasmid #13657)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 13657 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    src K295R Y527F
  • Alt name
    c-src
  • Alt name
    src
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2000
  • Mutation
    K295R Y527F. Dominant negative mutant.
  • Entrez Gene
    Src (a.k.a. AW259666, pp60c, pp60c-src)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

* The depositing lab is not completely sure of the backbone for this plasmid; Addgene's sequencing results indicate that it may instead be pcDNA3. The plasmid should still function correctly, but users should consult Addgene's sequencing results and exercise caution if subcloning is necessary.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV5 mouse src K295R Y527F was a gift from Joan Brugge & Peter Howley (Addgene plasmid # 13657 ; http://n2t.net/addgene:13657 ; RRID:Addgene_13657)