pLNCX chick src Y527F
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13660||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerBD Biosciences
- Backbone size w/o insert (bp) 6600
Vector typeMammalian Expression, Retroviral
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namesrc Y527F
SpeciesG. gallus (chicken)
Insert Size (bp)1900
MutationY527F. Constitutive active mutant.
Entrez GeneSRC (a.k.a. PP60C-SCR, SDR, c-src)
- Cloning method Restriction Enzyme
- 5′ cloning site ClaI (not destroyed)
- 3′ cloning site ClaI (not destroyed)
- 5′ sequencing primer LNCX primer (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLNCX chick src Y527F was a gift from Joan Brugge (Addgene plasmid # 13660 ; http://n2t.net/addgene:13660 ; RRID:Addgene_13660)