pLNCX chick src Y527F
(Plasmid #13660)

• Depositing Lab: Joan Brugge
• Publication: src plasmids (unpublished)

Backbone

• Vector backbone: pLNCX
• Backbone manufacturer: BD Biosciences
• Backbone size w/o insert (bp): 6600
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: src Y527F
• Alt name: src
• Species: G. gallus (chicken)
• Insert Size (bp): 1900
• Mutation: Y527F. Constitutive active mutant.
• Entrez Gene: SRC (a.k.a. PP60C-SCR, SDR, c-src)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ClaI (not destroyed)
• 3′ cloning site: ClaI (not destroyed)
• 5′ sequencing primer: LNCX primer

Resource Information

• Articles Citing this Plasmid:
  • 23 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry