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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 13659 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepLNCX
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Backbone manufacturerBD Biosciences
- Backbone size w/o insert (bp) 6600
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Vector typeMammalian Expression, Retroviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesrc K295R
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Alt namesrc
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SpeciesG. gallus (chicken)
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Insert Size (bp)1900
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MutationK295R. Kinase dead mutant.
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Entrez GeneSRC (a.k.a. PP60C-SCR, SDR, c-src)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site ClaI (not destroyed)
- 3′ cloning site ClaI (not destroyed)
- 5′ sequencing primer LNCX primer (Common Sequencing Primers)
Resource Information
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Terms and Licenses
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Industry Terms
- Not Available to Industry
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Articles Citing this Plasmid
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLNCX chick src K295R was a gift from Joan Brugge (Addgene plasmid # 13659 ; http://n2t.net/addgene:13659 ; RRID:Addgene_13659)
Map uploaded by the depositor.
