pLNCX chick src K295R
(Plasmid #13659)

• Depositing Lab: Joan Brugge
• Publication: src plasmids (unpublished)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	13659	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pLNCX
  (Search Vector Database)
• Backbone manufacturer: BD Biosciences
• Backbone size w/o insert (bp): 6600
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: src K295R
• Alt name: src
• Species: G. gallus (chicken)
• Insert Size (bp): 1900
• Mutation: K295R. Kinase dead mutant.
• Entrez Gene: SRC (a.k.a. PP60C-SCR, SDR, c-src)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ClaI (not destroyed)
• 3′ cloning site: ClaI (not destroyed)
• 5′ sequencing primer: LNCX primer

Resource Information

• Terms and Licenses:
  • UBMTA
• Industry Terms:
  • Not Available to Industry
• Articles Citing this Plasmid:
  • 5 References