|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17686||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6400
Vector typeMammalian Expression
Growth in Bacteria
Copy numberLow Copy
Gene/Insert namec-src Y529F
SpeciesM. musculus (mouse)
MutationTyr 529 changed to Phe .
Entrez GeneSrc (a.k.a. AW259666, pp60c, pp60c-src)
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pM5MF529 was a gift from David Shalloway (Addgene plasmid # 17686 ; http://n2t.net/addgene:17686 ; RRID:Addgene_17686)
For your References section:Transformation of mouse cells by wild-type mouse c-Src. Lin PH, Shenoy S, Galitski T, Shalloway D. Oncogene. 1995 Jan 19. 10(2):401-5. PubMed 7530829