pCI-neo:alpha4-eGFP
(Plasmid
#45068)
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Depositing Lab
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Depositing OrganizationCalifornia Institute of Technology
Located in United States of America -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 45068 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 45068-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 45068-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCI-Neo
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 5472
- Total vector size (bp) 8114
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namealpha4-eGFP
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Alt namenAChR alpha4-eGFP
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SpeciesM. musculus (mouse)
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Entrez GeneChrna4 (a.k.a. Acra-4, Acra4, EBN1, ENFL1)
- Promoter CMV
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Tags
/ Fusion Proteins
- Green Fluorescent Protien
- HA Tag
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byJ. Stizel (University of Colorado, Boulder, CO)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
α4 cloned into EcoRI sites of pCI-neo
DNA (Catalog # 45068-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 45068-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCI-neo:alpha4-eGFP was a gift from Henry Lester (Addgene plasmid # 45068 ; http://n2t.net/addgene:45068 ; RRID:Addgene_45068) -
For your References section:
Nicotine up-regulates alpha4beta2 nicotinic receptors and ER exit sites via stoichiometry-dependent chaperoning. Srinivasan R, Pantoja R, Moss FJ, Mackey ED, Son CD, Miwa J, Lester HA. J Gen Physiol. 2011 Jan;137(1):59-79. 10.1085/jgp.201010532 PubMed 21187334
