pCI-neoBeta2mcherry
(Plasmid #45097)

• Depositing Lab: Henry Lester
• Publication: Srinivasan et al J Gen Physiol. 2011 Jan;137(1):59-79.

Backbone

• Vector backbone: pCI-neo
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5472
• Total vector size (bp): 7731
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: nAChR beta2 mcherry
• Alt name: b2-mcherry
• Species: M. musculus (mouse)
• Insert Size (bp): 2259
• Mutation: β2 was cloned into pCI-neo at the restriction site EcoRI
• Entrez Gene: Chrnb2 (a.k.a. Acrb-2, Acrb2, C030030P04Rik, [b]2-nAchR)
• Promoter: CMV
• Tag / Fusion Protein:
  • has c-myc tag

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: J. Stizel (University of Colorado, Boulder, CO)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

PI: Henry A. Lester

β2 was cloned into pCI-neo at the restriction site EcoRI

Please note that the full sequence is for quick reference only. Please see Addgene's quality control sequence for the exact sequence of important regions.