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pEGFP-TRIM28
(Plasmid #45568)

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Item Catalog # Description Quantity Price (USD)
Plasmid 45568 Standard format: Plasmid sent in bacteria as agar stab 1 $94 *
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DNA 45568-D50 50 μg of DNA in Tris buffer $495 *
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DNA 45568-D100 100 μg of DNA in Tris buffer $585 *
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Backbone

  • Vector backbone
    pEGFP-C
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 7200
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TRIM28
  • Alt name
    tripartite motif containing 28
  • Alt name
    KAP1
  • Alt name
    TIF1β
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2500
  • Mutation
    G34S mutation in EGFP
  • GenBank ID
    NP_005753.1
  • Entrez Gene
    TRIM28 (a.k.a. KAP1, PPP1R157, RNF96, TF1B, TIF1B, TIF1beta)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (destroyed during cloning)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer EGFP-C; CMV-F
  • 3′ sequencing primer SV40-pA-R
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Full-length human TRIM28 was purchased from Origene and subcloned into vector pKH3 to generate pKH3-Trim28 (Addgene plasmid #45569). A full length TRIM28 DNA fragment was then generated from pKH3-TRIM28 by PCR and cloned into pEGFP.

The depositing laboratory indicated that the G34S mutation in EGFP does not affect the function of the EGFP-TRIM28 fusion protein.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-TRIM28 was a gift from Fanxiu Zhu (Addgene plasmid # 45568 ; http://n2t.net/addgene:45568 ; RRID:Addgene_45568)

  • For your References section:

    Tripartite motif-containing protein 28 is a small ubiquitin-related modifier E3 ligase and negative regulator of IFN regulatory factor 7. Liang Q, Deng H, Li X, Wu X, Tang Q, Chang TH, Peng H, Rauscher FJ 3rd, Ozato K, Zhu F. J Immunol. 2011 Nov 1;187(9):4754-63. doi: 10.4049/jimmunol.1101704. Epub 2011 Sep 21. 10.4049/jimmunol.1101704 PubMed 21940674
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