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pcDNA4 huZAP(L)
(Plasmid #45907)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45907 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 45907-D50 50 μg of DNA in Tris buffer $495
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DNA 45907-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pcDNA4
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ZAP(L) isoform
  • Alt name
    ZAP
  • Alt name
    ZC3HAV1 zinc finger CCCH-type, antiviral 1
  • Species
    H. sapiens (human)
  • Mutation
    M202T, L388F, Q701E, T851I (reference NP_064504.2)
  • Entrez Gene
    ZC3HAV1 (a.k.a. ARTD13, FLB6421, PARP13, ZAP, ZC3H2, ZC3HDC2)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site N/A (unknown if destroyed)
  • 3′ cloning site N/A (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-Rev
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There is a M202T amino acid change in hZAP(L) isoform, not known to effect function.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA4 huZAP(L) was a gift from Harmit Malik (Addgene plasmid # 45907 ; http://n2t.net/addgene:45907 ; RRID:Addgene_45907)

  • For your References section:

    Positive selection and increased antiviral activity associated with the PARP-containing isoform of human zinc-finger antiviral protein. Kerns JA, Emerman M, Malik HS. PLoS Genet. 2008 Jan;4(1):e21. doi: 10.1371/journal.pgen.0040021. 10.1371/journal.pgen.0040021 PubMed 18225958
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