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STAT3(Y705F)-TAL-Luc
(Plasmid #46933)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 46933 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 46933-D50 50 μg of DNA in Tris buffer $495
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DNA 46933-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pTAL-Luc
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 2351
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    STAT3(Y705F)
  • Alt name
    STAT3
  • Alt name
    signal transducer and activator of transcription 3 (acute-phase response factor)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2351
  • Mutation
    Tyrosine 705 to Phenylalanine
  • Entrez Gene
    STAT3 (a.k.a. ADMIO, ADMIO1, APRF, HIES)
  • Promoter TA Luciferase
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer 5'-CGGGAGGTACTTGGAGCG-3'
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    George Stark Lab, Case Western Reserve University

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    STAT3(Y705F)-TAL-Luc was a gift from Afshin Dowlati (Addgene plasmid # 46933 ; http://n2t.net/addgene:46933 ; RRID:Addgene_46933)

  • For your References section:

    The association and nuclear translocation of the PIAS3-STAT3 complex is ligand and time dependent. Dabir S, Kluge A, Dowlati A. Mol Cancer Res. 2009 Nov;7(11):1854-60. doi: 10.1158/1541-7786.MCR-09-0313. Epub 2009 Nov 10. 10.1158/1541-7786.MCR-09-0313 PubMed 19903771
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