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Stat3 Y705F Flag pRc/CMV
(Plasmid #8709)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8709 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRc/CMV
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Stat3 Y705F
  • Alt name
    Stat3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2600
  • Mutation
    Y705F
  • GenBank ID
    U06922
  • Entrez Gene
    Stat3 (a.k.a. 1110034C02Rik, A, AW109958, Aprf)
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site ApaI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

Diagnostic Restriction Digest with BamHI (3.2kb, 2.8kb, and 2.0kb). Due to a stop codon error made when tagging the Stat3 clone with FLAG (DYKDDDDK) additional residues follow the tag (GPYSIVSPKC STOP). The ApaI cloning site is GGG CCC, which encodes the G and P residues coming after the FLAG tag. Therefore, if the insert is removed with a NotI and ApaI digestion, it will not have a stop codon included.

* E16K mutation found during QC

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Stat3 Y705F Flag pRc/CMV was a gift from Jim Darnell (Addgene plasmid # 8709 ; http://n2t.net/addgene:8709 ; RRID:Addgene_8709)
  • For your References section:

    Mapping of Stat3 serine phosphorylation to a single residue (727) and evidence that serine phosphorylation has no influence on DNA binding of Stat1 and Stat3. Wen Z, Darnell JE Jr. Nucleic Acids Res 1997 Jun 1;25(11):2062-7. 10.1093/nar/25.11.2062 PubMed 9153303