psicheck-2-Tas2r3
(Plasmid
#48176)
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Purposecontains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element
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Depositing Lab
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Depositing OrganizationMassachusetts General Hospital
Located in United States of America -
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 48176 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 48176-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 48176-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepsicheck2
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 6273
- Total vector size (bp) 6339
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Vector typeLuciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name5" UTR of TAS2R3
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SpeciesH. sapiens (human)
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Insert Size (bp)66
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GenBank IDNM_016943
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Entrez GeneTAS2R3 (a.k.a. T2R3)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Nhe1 (not destroyed)
- 3′ cloning site Nhe1 (not destroyed)
- 5′ sequencing primer T7
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 48176-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 48176-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
psicheck-2-Tas2r3 was a gift from Vamsi Mootha (Addgene plasmid # 48176 ; http://n2t.net/addgene:48176 ; RRID:Addgene_48176) -
For your References section:
Upstream open reading frames cause widespread reduction of protein expression and are polymorphic among humans. Calvo SE, Pagliarini DJ, Mootha VK. Proc Natl Acad Sci U S A. 2009 May 5;106(18):7507-12. doi: 10.1073/pnas.0810916106. Epub 2009 Apr 16. 10.1073/pnas.0810916106 PubMed 19372376