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8xLexA-binding elements/pMD20
(Plasmid #48807)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 48807 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMD20
  • Backbone manufacturer
    Takara Bio
  • Backbone size w/o insert (bp) 2736
  • Total vector size (bp) 2951

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    8xLexA-binding elements
  • Alt name
    8xLexA BE
  • Species
    Synthetic
  • Insert Size (bp)
    170

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Kpn I (not destroyed)
  • 3′ cloning site Xho I (not destroyed)
  • 5′ sequencing primer M13 primer M4
  • 3′ sequencing primer M13 primer RV
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid

Depositor Comments

For more information on Fujii Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/fujii/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    8xLexA-binding elements/pMD20 was a gift from Hodaka Fujii (Addgene plasmid # 48807 ; http://n2t.net/addgene:48807 ; RRID:Addgene_48807)
  • For your References section:

    Direct identification of insulator components by insertional chromatin immunoprecipitation. Fujita T, Fujii H. PLoS One. 2011;6(10):e26109. doi: 10.1371/journal.pone.0026109. Epub 2011 Oct 17. 10.1371/journal.pone.0026109 PubMed 22043306