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(Plasmid #48874)


Item Catalog # Description Quantity Price (USD)
Plasmid 48874 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4605
  • Total vector size (bp) 5352
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    3xFLAG-NLS-LexA binding domain
  • Species
  • Insert Size (bp)
  • Promoter CMV promoter
  • Tags / Fusion Proteins
    • 3xFLAG tag (N terminal on insert)
    • NLS (nuclear localization signal) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH I (destroyed during cloning)
  • 3′ cloning site Sal I (not destroyed)
  • 5′ sequencing primer AAT GTC GTA ATA ACC CCG CCC CGT TGA CGC
  • 3′ sequencing primer TAT TAG GAC AAG GCT GGT GGG CAC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid is described in the following article:

Efficient isolation of specific genomic regions by insertional chromatin immunoprecipitation (iChIP) with a second-generation tagged LexA DNA-binding domain.

Toshitsugu Fujita, Hodaka Fujii

Advances in Bioscience and Biotechnology, 2013
DOI: 10.4236/abb.2012.35081

For more information on Fujii Lab CRISPR Plasmids please refer to:

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3xFNLDD/pCMV-7.1 was a gift from Hodaka Fujii (Addgene plasmid # 48874 ; ; RRID:Addgene_48874)
  • For your References section:

    A Critical Role of the Thy28-MYH9 Axis in B Cell-Specific Expression of the Pax5 Gene in Chicken B Cells. Fujita T, Kitaura F, Fujii H. PLoS One. 2015 Jan 21;10(1):e0116579. doi: 10.1371/journal.pone.0116579. eCollection 2015. PONE-D-14-45451 [pii] PubMed 25607658