Purpose(Empty Backbone) for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences into the S. pombe urg1 locus
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||48989||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
/ Fusion Protein
- 3XHA tag (C terminal on backbone)
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
Terms and Licenses
This plasmid contains a wild-type loxP site and a mutant loxP site termed loxM3. Addgene's mapping software can not differentiate between the two sites and identifies the loxM3 site as loxP. The locations of the loxP and loxM3 sites are:
loxP = 3859-3892
loxM3 = 4131-4164
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAW8ENdeI-c3HA was a gift from Tony Carr (Addgene plasmid # 48989 ; http://n2t.net/addgene:48989 ; RRID:Addgene_48989)
For your References section:Optimisation of the Schizosaccharomyces pombe urg1 Expression System. Watson AT, Daigaku Y, Mohebi S, Etheridge TJ, Chahwan C, Murray JM, Carr AM. PLoS One. 2013 Dec 20;8(12):e83800. doi: 10.1371/journal.pone.0083800. PubMed 24376751