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Optimisation of the Schizosaccharomyces pombe urg1 Expression System.
Watson AT, Daigaku Y, Mohebi S, Etheridge TJ, Chahwan C, Murray JM, Carr AM
PLoS One. 2013 Dec 20;8(12):e83800. doi: 10.1371/journal.pone.0083800.
PubMed

Plasmids from Article

ID Plasmid Purpose  
48975pAW8ENdeIfor Cre-lox cassette exchange of untagged gene sequences into the S. pombe urg1 locus
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48976pAW8ENdeI-3XDSRfor Cre-lox cassette exchange of untagged gene sequences together with 3 copies of the core DSR motif into the S. pombe urg1 locus
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48978pAW8ENdeI-4XDSRfor Cre-lox cassette exchange of untagged gene sequences together with 4 copies of the core DSR motif into the S. pombe urg1 locus
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48980pAW8ENdeI-6XDSRfor Cre-lox cassette exchange of untagged gene sequences together with 6 copies of the core DSR motif into the S. pombe urg1 locus
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48981pAW8ENdeI-8XDSRfor Cre-lox cassette exchange of untagged gene sequences together with 8 copies of the core DSR motif into the S. pombe urg1 locus
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48983pAW8ENdeI-spo5DSRfor Cre-lox cassette exchange of untagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locus
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48988pAW8ENdeI-cyEGFPfor Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences into the S. pombe urg1 locus
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48989pAW8ENdeI-c3HAfor Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences into the S. pombe urg1 locus
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48990pAW8ENdeI-cHAIAA17for Cre-lox cassette exchange of C-terminal HA-auxin-inducible degron tagged gene sequences into the S. pombe urg1 locus
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49863pAW8ENdeI-cyEGFP-3XDSRfor Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with 3 copies of the core DSR motif into the S. pombe urg1 locus
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49864pAW8ENdeI-cyEGFP-4XDSRfor Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with 4 copies of the core DSR motif into the S. pombe urg1 locus
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49865pAW8ENdeI-cyEGFP-6XDSRfor Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with 6 copies of the core DSR motif into the S. pombe urg1 locus
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49866pAW8ENdeI-cyEGFP-8XDSRfor Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with 8 copies of the core DSR motif into the S. pombe urg1 locus
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49867pAW8ENdeI-cyEGFP-spo5DSRfor Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locus
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49868pAW8ENdeI-c3HA-3XDSRfor Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with 3 copies of the core DSR motif into the S. pombe urg1 locus
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49869pAW8ENdeI-c3HA-4XDSRfor Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with 4 copies of the core DSR motif into the S. pombe urg1 locus
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49870pAW8ENdeI-c3HA-6XDSRfor Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with 6 copies of the core DSR motif into the S. pombe urg1 locus
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49871pAW8ENdeI-c3HA-8XDSRfor Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with 8 copies of the core DSR motif into the S. pombe urg1 locus
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49872pAW8ENdeI-c3HA-spo5DSRfor Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locus
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49873pAW8ENdeI-cHAIAA7-spo5DSRfor Cre-lox cassette exchange of C-terminal HA-auxin-inducible degron tagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locus
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