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pCMV5-hBMPR-1A CA
(Plasmid #49527)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49527 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 49527-D50 50 μg of DNA in Tris buffer $495
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DNA 49527-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCMV5
  • Backbone manufacturer
    Dr. David Russell, UTSW
  • Backbone size w/o insert (bp) 4657
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    BMPR-1A CA
  • Alt name
    BMPR-1A
  • Alt name
    Alk3
  • Species
    H. sapiens (human)
  • Mutation
    constitutively active mutation (Q233D); also P2A and K208R
  • Entrez Gene
    BMPR1A (a.k.a. 10q23del, ACVRLK3, ALK-3, ALK3, BMPR-1A, CD292, SKR5)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer hGH-PA-R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The BMPR-1A insert in this plasmid contains P2A and K208R mutations when compared to GenBank reference sequence NP_004320.2. These mutations are not a concern for the function of the plasmid, as described in the associated publication.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV5-hBMPR-1A CA was a gift from Lee Niswander & Peter ten Dijke (Addgene plasmid # 49527 ; http://n2t.net/addgene:49527 ; RRID:Addgene_49527)

  • For your References section:

    Expression of a constitutively active type I BMP receptor using a retroviral vector promotes the development of adrenergic cells in neural crest cultures. Varley JE, McPherson CE, Zou H, Niswander L, Maxwell GD. Dev Biol. 1998 Apr 1;196(1):107-18. 10.1006/dbio.1998.8853 PubMed 9527884
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