pME-puro-SNAP-FLAG-CD59
(Plasmid #50374)

• Purpose: Express SNAP and FLAG-tagged human CD59, a GPI-AP in mammmalian cells
• Depositing Lab: Reika Watanabe
• Publication: Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33. doi: 10.1091/mbc.E13-03-0160. Epub 2013 Apr 24.

Backbone

• Vector backbone: pME-puro-FLAG-CD59
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 6094
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SNAP
• Species: Synthetic
• Insert Size (bp): 546
• Promoter: SR alpha

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SbfI (not destroyed)
• 3′ cloning site: SbfI (not destroyed)
• 5′ sequencing primer: atg gac aaa gac tgc gaa atg
• 3′ sequencing primer: acc cag ccc agg ctt gcc cag

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Prof. Kai Johnsson at the Institute of Chemical Sciences and Engineering of the École Polytechnique Fédérale de Lausanne (EPFL), Switzerland
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry