Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||25630||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5369
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberLow Copy
SpeciesM. musculus (mouse)
Insert Size (bp)1476
Entrez GeneNampt (a.k.a. 1110035O14Rik, AI314458, AI480535, NAmPRTase, Pbef, Pbef1, Visfatin)
/ Fusion Protein
- His6 (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7term (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Nampt-PET28a was a gift from Cynthia Wolberger (Addgene plasmid # 25630 ; http://n2t.net/addgene:25630 ; RRID:Addgene_25630)
For your References section:Structure of Nampt/PBEF/visfatin, a mammalian NAD+ biosynthetic enzyme. Wang T, Zhang X, Bheda P, Revollo JR, Imai S, Wolberger C. Nat Struct Mol Biol. 2006 Jul . 13(7):661-2. 10.1038/nsmb1114 PubMed 16783373