|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26161||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4373
Vector typeBacterial Expression
Growth in Bacteria
Copy numberLow Copy
Gene/Insert nameBacterial luciferase operon + G13 promoter
SpeciesMycobacterium marinum + Photorhabdus luminescens
Insert Size (bp)6186
MutationIt contains a gram-positive enhanced translation signal in front of luxA, luxC and luxE. G13 promoter cloned in front of luxC
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRV (destroyed during cloning)
- 5′ sequencing primer agaataacgttggcactcgc (Common Sequencing Primers)
The sequence of the Lux operon is theoretical. As such, there are a few differences between Addgene sequence and full plasmid sequence. These differences do not affect plasmid function.
Qazi, S.N., et al., agr expression precedes escape of internalized Staphylococcus aureus from the host endosome. Infect Immun, 2001. 69(11): p. 7074-82.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMV306hsp+LuxG13 was a gift from Brian Robertson & Siouxsie Wiles (Addgene plasmid # 26161 ; http://n2t.net/addgene:26161 ; RRID:Addgene_26161)
For your References section:Optimisation of bioluminescent reporters for use with mycobacteria. Andreu N, Zelmer A, Fletcher T, Elkington PT, Ward TH, Ripoll J, Parish T, Bancroft GJ, Schaible U, Robertson BD, Wiles S. PLoS One. 2010 May 24;5(5):e10777. 10.1371/journal.pone.0010777 PubMed 20520722