Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14073||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4950
Vector typeBacterial Expression
Growth in Bacteria
Copy numberLow Copy
Gene/Insert nameBacterial luciferase
Insert Size (bp)6954
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1 (not destroyed)
- 3′ cloning site EcoR1 (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAKlux1 was a gift from Attila Karsi (Addgene plasmid # 14073 ; http://n2t.net/addgene:14073 ; RRID:Addgene_14073)
For your References section:Development of bioluminescent Edwardsiella ictaluri for noninvasive disease monitoring. Karsi A, Menanteau-Ledouble S, Lawrence ML. FEMS Microbiol Lett. 2006 Jul . 260(2):216-23. 10.1111/j.1574-6968.2006.00310.x PubMed 16842347