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hIR-GFP
(Plasmid #22286)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 22286 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-N2
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    INSR insulin receptor
  • Alt name
    IR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4700
  • GenBank ID
    M10051
  • Entrez Gene
    INSR (a.k.a. CD220, HHF5)
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (destroyed during cloning)
  • 3′ cloning site ApaI (not destroyed)
  • 5′ sequencing primer ACG CAA ATG GGC GGT AGG CG
  • 3′ sequencing primer TCG CCG GAC ACG CTG AAC TTG TG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hIR-GFP was a gift from Joseph Bass (Addgene plasmid # 22286 ; http://n2t.net/addgene:22286 ; RRID:Addgene_22286)
  • For your References section:

    Calreticulin and Hsp90 stabilize the human insulin receptor and promote its mobility in the endoplasmic reticulum. Ramos RR, Swanson AJ, Bass J. Proc Natl Acad Sci U S A. 2007 Jun 19. 104(25):10470-5. 10.1073/pnas.0701114104 PubMed 17563366