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pBABE puro mouse IRS-1 myc
(Plasmid #11374)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11374 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBABE puro
  • Backbone manufacturer
    pBABE-puro is available at Addgene
  • Backbone size w/o insert (bp) 5169
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IRS-1
  • Alt name
    Insulin Receptor Substrate 1
  • Alt name
    IRS1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3740
  • GenBank ID
    NM_010570
  • Entrez Gene
    Irs1 (a.k.a. G972R, IRS-1)
  • Tag / Fusion Protein
    • myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer pBABE 5
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

(Kahn lab plasmid name = pBabe puro IRS-1 Myc)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABE puro mouse IRS-1 myc was a gift from Ronald Kahn (Addgene plasmid # 11374 ; http://n2t.net/addgene:11374 ; RRID:Addgene_11374)
  • For your References section:

    Insulin receptor substrate 3 (IRS-3) and IRS-4 impair IRS-1- and IRS-2-mediated signaling. Tsuruzoe K, Emkey R, Kriauciunas KM, Ueki K, Kahn CR. Mol Cell Biol. 2001 Jan . 21(1):26-38. 10.1128/MCB.21.1.26-38.2001 PubMed 11113178