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pBABE-bleo IGF-1R
(Plasmid #11212)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11212 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBabe-bleo
  • Backbone size w/o insert (bp) 4888
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IGF-1R
  • Alt name
    IGFR
  • Alt name
    IGF-I receptor
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4200
  • Entrez Gene
    IGF1R (a.k.a. CD221, IGFIR, IGFR, JTK13)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Eva Surmacz
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

The insert was cloned from pcDNA IGF-1R (which contains a 4.2kb IGF-1R insert that lacks about 700bp of 3' sequence. This insert was cloned into the XhoI-XbaI sites of pcDNA). An EcoRI/XbaI fragment of pcDNA-IGF-1R was then inserted into SnaBI site of pBABE-bleo (EcoRI and XbaI sites were blunted).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABE-bleo IGF-1R was a gift from Ronald Kahn (Addgene plasmid # 11212 ; http://n2t.net/addgene:11212 ; RRID:Addgene_11212)
  • For your References section:

    Differential roles of the insulin and insulin-like growth factor-I (IGF-I) receptors in response to insulin and IGF-I. Entingh-Pearsall A, Kahn CR. J Biol Chem. 2004 Sep 3. 279(36):38016-24. 10.1074/jbc.M313201200 PubMed 15247278