pLenti-ReaChR-citrine
(Plasmid #50956)

• Purpose: ReaChR-citrine in lentival transfer vector under human synapsin promoter
• Depositing Lab: Roger Tsien
• Publication: Lin et al Nat Neurosci. 2013 Oct;16(10):1499-508. doi: 10.1038/nn.3502. Epub 2013 Sep 1.

Backbone

• Vector backbone: AAV2
• Backbone manufacturer: Deisseroth / Boyden lab
• Backbone size w/o insert (bp): 8451
• Total vector size (bp): 10227
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: Due to the lentiviral long terminal repeats, using Stbl3 and Stbl2 cells would minimize the recombination event. We have not observed recombination with regular DH5alpha in our experience.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: ReaChR-citrine
• Species: Synthetic
• Insert Size (bp): 1776
• GenBank ID: KF448069
• Promoter: human synapsin promoter
• Tag / Fusion Protein:
  • citrine (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NheI, EcoRI (not destroyed)
• 5′ sequencing primer: none
• 3′ sequencing primer: none

Resource Information

• Supplemental Documents:
  • Genbank annotated sequence file
• A portion of this plasmid was derived from a plasmid made by: Plasmid vector was originally from Ed Boyden.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry