pME-nlsGal80(opt)
(Plasmid
#51618)
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Purposevertebrate expression of codon-optimized Gal80 with N-terminal NLS and no stop
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Depositing Lab
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Depositing OrganizationUniversity of Utah
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 51618 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 51618-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 51618-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backboneGateway middle entry clone (pME)
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Vector typevertebrate expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGal80(opt)
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Alt nameGal80
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SpeciesS. cerevisiae (budding yeast)
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Mutationcodon optimized
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Entrez GeneGAL80 (a.k.a. YML051W)
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Tag
/ Fusion Protein
- NLS (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13R
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pME-NLS-Gal80opt was generated by PCR using primers 5'-ATGGCTCCAAAGAAGAAGCGTAAGGTAATGGACTACAACAAAAGGAGCAG-3' for the nuclear localization signal (Kwan et al., 2007) and 5'-CAGTGAGTAGTGAGAGATATTTG-3' for codon-optimized Gal80 (GenScript; GenBank accession no. JN314417).
DNA (Catalog # 51618-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 51618-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pME-nlsGal80(opt) was a gift from Joshua Bonkowsky (Addgene plasmid # 51618 ; http://n2t.net/addgene:51618 ; RRID:Addgene_51618) -
For your References section:
Gal80 intersectional regulation of cell-type specific expression in vertebrates. Fujimoto E, Gaynes B, Brimley CJ, Chien CB, Bonkowsky JL. Dev Dyn. 2011 Oct;240(10):2324-34. doi: 10.1002/dvdy.22734. Epub 2011 Sep 8. 10.1002/dvdy.22734 PubMed 21905164
