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pME-Gal80ts
(Plasmid #51619)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 51619 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gal80ts
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    temperature sensitive (G203V, G323R)*
  • GenBank ID
  • Entrez Gene
    GAL80 (a.k.a. YML051W)

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

pME-Gal80ts was cloned from pBPHLWL-Gal80ts (McGuire et al., 2003) by PCR (kind gift of T. Clandinin).

*Also has a non-functional E101D mutation

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pME-Gal80ts was a gift from Joshua Bonkowsky (Addgene plasmid # 51619 ; http://n2t.net/addgene:51619 ; RRID:Addgene_51619)
  • For your References section:

    Gal80 intersectional regulation of cell-type specific expression in vertebrates. Fujimoto E, Gaynes B, Brimley CJ, Chien CB, Bonkowsky JL. Dev Dyn. 2011 Oct;240(10):2324-34. doi: 10.1002/dvdy.22734. Epub 2011 Sep 8. 10.1002/dvdy.22734 PubMed 21905164