M2660 ura3::LYS2 Disruptor Converter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||51678||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerMarsh et al., 1984
Vector typeYeast Expression ; yeast marker swap
Growth in Bacteria
SpeciesS. cerevisiae (budding yeast)
Entrez GeneLYS2 (a.k.a. YBR115C)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRV (destroyed during cloning)
- 3′ cloning site PstI (not destroyed)
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Plasmid M2660 (ura3::LYS2 ) was made by cutting M581 with EcoRV and PstI and replacing the 208 bp EcoRV–PstI fragment with a 4.6 kb PvuII–PstI fragment containing LYS2 from plasmid YDp-K.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:M2660 ura3::LYS2 Disruptor Converter was a gift from David Stillman (Addgene plasmid # 51678 ; http://n2t.net/addgene:51678 ; RRID:Addgene_51678)
For your References section:New 'marker swap' plasmids for converting selectable markers on budding yeast gene disruptions and plasmids. Voth WP, Jiang YW, Stillman DJ. Yeast. 2003 Aug;20(11):985-93. 10.1002/yea.1018 PubMed 12898713