Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

M4755 KanMX::LEU2 Disruptor Converter
(Plasmid #51685)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 51685 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    M4757 (kanMX::TRP1), Addgene plasmid #51687
  • Vector type
    Yeast Expression ; yeast marker swap
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LEU2
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    LEU2 (a.k.a. YCL018W)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SphI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

M4756 (kanMX::LEU2)was made by digesting M4757 with BamHI and SphI and inserting a 2.0 kb BamHI–SphI fragment with LEU2 from plasmid pJJ250.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    M4755 KanMX::LEU2 Disruptor Converter was a gift from David Stillman (Addgene plasmid # 51685 ; http://n2t.net/addgene:51685 ; RRID:Addgene_51685)
  • For your References section:

    New 'marker swap' plasmids for converting selectable markers on budding yeast gene disruptions and plasmids. Voth WP, Jiang YW, Stillman DJ. Yeast. 2003 Aug;20(11):985-93. 10.1002/yea.1018 PubMed 12898713