M4757 KanMX::TRP1 Disruptor Converter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||51687||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepAG25 (Goldstein and McCusker, 1999)
Vector typeYeast Expression
Growth in Bacteria
SpeciesS. cerevisiae (budding yeast)
Entrez GeneTRP1 (a.k.a. YDR007W)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site SphI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
First, plasmid pAG25 (Goldstein and McCusker, 1999) was digested with HindIII and PacI, treated with Klenow and ligated closed. This plasmid was digested with KpnI and SphI and the 0.9 kb KpnI–SphI fragment containing TRP1 from pJJ246 was inserted, constructing M4757.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:M4757 KanMX::TRP1 Disruptor Converter was a gift from David Stillman (Addgene plasmid # 51687 ; http://n2t.net/addgene:51687 ; RRID:Addgene_51687)
For your References section:New 'marker swap' plasmids for converting selectable markers on budding yeast gene disruptions and plasmids. Voth WP, Jiang YW, Stillman DJ. Yeast. 2003 Aug;20(11):985-93. 10.1002/yea.1018 PubMed 12898713