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Purpose9 copies of NFAT sites in minimal alphaMHC-pGL-3
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Depositing Lab
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Depositing OrganizationCincinnati Children's Hospital Medical Center
Located in United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 51941 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 51941-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 51941-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepGL-3 Basic
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 4818
- Total vector size (bp) 5100
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Vector typeLuciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert name9x NFAT binding site
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Alt nameNine copies of an NFAT binding site from the IL-4 promoter (5′-TGGAAAATT-3′)
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Insert Size (bp)81
Cloning Information for Gene/Insert 1
- Cloning method Unknown
- 5′ sequencing primer RVprimer3
- 3′ sequencing primer na
- (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert namealpha-MHC
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Alt nameminimal promoter from the α-myosin heavy chain gene (−164 to +16)
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Insert Size (bp)180
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Tag
/ Fusion Protein
- luciferase (C terminal on backbone)
Cloning Information for Gene/Insert 2
- Cloning method Unknown
- 5′ sequencing primer na
- 3′ sequencing primer LucNRev
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 51941-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 51941-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
9X NFAT-apha-MHC-Luc was a gift from Jeffery Molkentin (Addgene plasmid # 51941 ; http://n2t.net/addgene:51941 ; RRID:Addgene_51941) -
For your References section:
Calcineurin/NFAT coupling participates in pathological, but not physiological, cardiac hypertrophy. Wilkins BJ, Dai YS, Bueno OF, Parsons SA, Xu J, Plank DM, Jones F, Kimball TR, Molkentin JD. Circ Res. 2004 Jan 9;94(1):110-8. Epub 2003 Dec 1. 10.1161/01.RES.0000109415.17511.18 PubMed 14656927
Map uploaded by the depositor.
