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pLV hUbC-Cas9-T2A-GFP Notes

Addgene diagnostic digest

This gel depicts the NEB 1kb Extend DNA ladder and a digestion of plasmid 53190 with KpnI-HF, AgeI-HF, and BamHI-HF.

We expected the following banding patterns:

KpnI-HF single digest: Bands at 7.4, 5.8 and 1.5kb

BamHI-HF single digest: Linearized plasmid ~15kb

AgeI-HF/BamHI-HF double digest: Bands at 12 and 3kb