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Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector.
Kabadi AM, Ousterout DG, Hilton IB, Gersbach CA
Nucleic Acids Res. 2014 Aug 13. pii: gku749.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
53186phH1-gRNAExpresses the S. pyogenes sgRNA from the H1 promoter
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53187pmU6-gRNAExpresses the S. pyogenes sgRNA from the mouse U6 promoter
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53188phU6-gRNAExpresses the S. pyogenes sgRNA from the human U6 promoter
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53189ph7SK-gRNAExpresses the S. pyogenes sgRNA from the 7SK promoter
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53190pLV hUbC-Cas9-T2A-GFP3rd generation transfer vector. Co-expresses human optimized S. pyogenes Cas9 and GFP
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53191pLV hUbC-dCas9-T2A-GFPCo-expresses human optimized S. pyogenes dCas9 and GFP
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53192pLV hUbC-dCas9 VP64-T2A-GFPCo-expresses human optimized S. pyogenes dCas9-VP64 and GFP
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59791pLV hUbC-VP64 dCas9 VP64-T2A-GFPCo-expresses human optimized S. pyogenes dCas9 fused to two copies of VP64 and GFP
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67620pLV hUbC-dCas9 KRAB-T2A-GFPCo-expressed human optimized S. pyogenese dCas9 fused to KRAB repressor domain and GFP
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84034pLV GG hUbC-dsREDContains Golden Gate cassette to express up to four gRNAs and dsRED
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