PurposeCo-expresses human optimized S. pyogenes dCas9 and GFP
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||53191||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 10000
- Total vector size (bp) 15000
Vector typeMammalian Expression, Lentiviral, CRISPR
Selectable markersZeo marker is outside the LTRs and will not be packaged into virus.
Growth in Bacteria
Gene/Insert namehumanized dead Cas9 T2A GFP
MutationD10A and H840A
- Promoter hUbC
/ Fusion Protein
- Flag (N terminal on insert)
- Cloning method Unknown
- 5′ sequencing primer hUBCpro-F
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLV hUbC-dCas9-T2A-GFP was a gift from Charles Gersbach (Addgene plasmid # 53191 ; http://n2t.net/addgene:53191 ; RRID:Addgene_53191)
For your References section:Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector. Kabadi AM, Ousterout DG, Hilton IB, Gersbach CA. Nucleic Acids Res. 2014 Aug 13. pii: gku749. 10.1093/nar/gku749 PubMed 25122746