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pLV hUbC-dCas9-T2A-GFP Citations (12)

Originally described in: Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector.
Kabadi AM, Ousterout DG, Hilton IB, Gersbach CA Nucleic Acids Res. 2014 Aug 13. pii: gku749.
PubMed Journal

Articles Citing pLV hUbC-dCas9-T2A-GFP

Highly specific epigenome editing by CRISPR-Cas9 repressors for silencing of distal regulatory elements. Thakore PI, D'Ippolito AM, Song L, Safi A, Shivakumar NK, Kabadi AM, Reddy TE, Crawford GE, Gersbach CA. Nat Methods. 2015 Dec;12(12):1143-9. doi: 10.1038/nmeth.3630. Epub 2015 Oct 26. PubMed

Associated Plasmids

The GPSM2/LGN GoLoco motifs are essential for hearing. Bhonker Y, Abu-Rayyan A, Ushakov K, Amir-Zilberstein L, Shivatzki S, Yizhar-Barnea O, Elkan-Miller T, Tayeb-Fligelman E, Kim SM, Landau M, Kanaan M, Chen P, Matsuzaki F, Sprinzak D, Avraham KB. Mamm Genome. 2016 Feb;27(1-2):29-46. doi: 10.1007/s00335-015-9614-7. Epub 2015 Dec 11. PubMed
N-cadherin is Key to Expression of the Nucleus Pulposus Cell Phenotype under Selective Substrate Culture Conditions. Hwang PY, Jing L, Chen J, Lim FL, Tang R, Choi H, Cheung KM, Risbud MV, Gersbach CA, Guilak F, Leung VY, Setton LA. Sci Rep. 2016 Jun 13;6:28038. doi: 10.1038/srep28038. PubMed
Targeted DNA methylation in vivo using an engineered dCas9-MQ1 fusion protein. Lei Y, Zhang X, Su J, Jeong M, Gundry MC, Huang YH, Zhou Y, Li W, Goodell MA. Nat Commun. 2017 Jul 11;8:16026. doi: 10.1038/ncomms16026. PubMed

Associated Plasmids

Distinct roles for phosphoinositide 3-kinases gamma and delta in malignant B cell migration. Ali AY, Wu X, Eissa N, Hou S, Ghia JE, Murooka TT, Banerji V, Johnston JB, Lin F, Gibson SB, Marshall AJ. Leukemia. 2018 Sep;32(9):1958-1969. doi: 10.1038/s41375-018-0012-5. Epub 2018 Jan 31. PubMed
Reduction of Kaposi's Sarcoma-Associated Herpesvirus Latency Using CRISPR-Cas9 To Edit the Latency-Associated Nuclear Antigen Gene. Tso FY, West JT, Wood C. J Virol. 2019 Mar 21;93(7). pii: JVI.02183-18. doi: 10.1128/JVI.02183-18. Print 2019 Apr 1. PubMed
Identification of a pituitary ERalpha-activated enhancer triggering the expression of Nr5a1, the earliest gonadotrope lineage-specific transcription factor. Pacini V, Petit F, Querat B, Laverriere JN, Cohen-Tannoudji J, L'hote D. Epigenetics Chromatin. 2019 Aug 7;12(1):48. doi: 10.1186/s13072-019-0291-8. PubMed
Transcription Factor-Directed Re-wiring of Chromatin Architecture for Somatic Cell Nuclear Reprogramming toward trans-Differentiation. Dall'Agnese A, Caputo L, Nicoletti C, di Iulio J, Schmitt A, Gatto S, Diao Y, Ye Z, Forcato M, Perera R, Bicciato S, Telenti A, Ren B, Puri PL. Mol Cell. 2019 Nov 7;76(3):453-472.e8. doi: 10.1016/j.molcel.2019.07.036. Epub 2019 Sep 10. PubMed
Artificial escape from XCI by DNA methylation editing of the CDKL5 gene. Halmai JANM, Deng P, Gonzalez CE, Coggins NB, Cameron D, Carter JL, Buchanan FKB, Waldo JJ, Lock SR, Anderson JD, O'Geen H, Segal DJ, Nolta J, Fink KD. Nucleic Acids Res. 2020 Mar 18;48(5):2372-2387. doi: 10.1093/nar/gkz1214. PubMed
DUX-miR-344-ZMYM2-Mediated Activation of MERVL LTRs Induces a Totipotent 2C-like State. Yang F, Huang X, Zang R, Chen J, Fidalgo M, Sanchez-Priego C, Yang J, Caichen A, Ma F, Macfarlan T, Wang H, Gao S, Zhou H, Wang J. Cell Stem Cell. 2020 Feb 6;26(2):234-250.e7. doi: 10.1016/j.stem.2020.01.004. PubMed
Unraveling the functional role of DNA demethylation at specific promoters by targeted steric blockage of DNA methyltransferase with CRISPR/dCas9. Sapozhnikov DM, Szyf M. Nat Commun. 2021 Sep 29;12(1):5711. doi: 10.1038/s41467-021-25991-9. PubMed

Associated Plasmids

Transcriptional regulation of Satb1 in mouse trophoblast stem cells. Yu W, Chakravarthi VP, Borosha S, Dilower I, Lee EB, Ratri A, Starks RR, Fields PE, Wolfe MW, Faruque MO, Tuteja G, Rumi MAK. Front Cell Dev Biol. 2022 Dec 14;10:918235. doi: 10.3389/fcell.2022.918235. eCollection 2022. PubMed

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