PurposeLentiviral expression of Cre recombinase and mCherry reporter from CMV promoter (separated by P2A peptide), and blasticidin resistance gene for selection in mammalian cells.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||179390||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6488
- Total vector size (bp) 9175
Vector typeMammalian Expression, Lentiviral, Cre/Lox
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Gene/Insert namemCherry (P2A) Cre recombinase
SpeciesH. sapiens (human), M. musculus (mouse), R. norvegicus (rat)
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site SacII (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made bypLM-CMV-R-Cre (Addgene Plasmid #27546)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please note: The plasmid contains an IS1 element insertion within a region of the plasmid near the origin of replication and outside of the region that will be packaged as part of the lentivirus particles to be produced from this plasmid. This insertion is not known to affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLenti-mCherry-Cre-blast was a gift from Moshe Szyf (Addgene plasmid # 179390 ; http://n2t.net/addgene:179390 ; RRID:Addgene_179390)
For your References section:Unraveling the functional role of DNA demethylation at specific promoters by targeted steric blockage of DNA methyltransferase with CRISPR/dCas9. Sapozhnikov DM, Szyf M. Nat Commun. 2021 Sep 29;12(1):5711. doi: 10.1038/s41467-021-25991-9. 10.1038/s41467-021-25991-9 PubMed 34588447