pCM42 (AAV-KPL) (AAV-(U6-sgRNA)x3 (sgKras.1, sgp53.8, sgLkb1.1)-pEFs-Rluc-P2A-Cre-shortPA--[Kras[G12D] HDR donor])
(Plasmid
#60172)
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PurposeMammalian expression vector containing: 1) sgRNAs targeting Kras, p53 and Lkb1, 2) Cre recombinase, 3) Renilla luciferase, and 4) Kras[G12D] HDR donor template.
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Depositing Labs
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 60172 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepAAV
- Total vector size (bp) 6988
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Vector typeMammalian Expression, Mouse Targeting, AAV, Cre/Lox, CRISPR, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameU6-sgRNA (Kras, p53, Lkb1), Renilla luciferase, Cre recombinase
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Insert Size (bp)4391
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCM42 (AAV-KPL) (AAV-(U6-sgRNA)x3 (sgKras.1, sgp53.8, sgLkb1.1)-pEFs-Rluc-P2A-Cre-shortPA--[Kras[G12D] HDR donor]) was a gift from (Addgene plasmid # 60172)
